Reducing Damage in Stroke

Purinergic Treatment in a Stroke Model Reduces Brain Lesion Size

Neuronal Lesion AreaThe middle cerebral artery of mice was occluded for 1 hr and then released (MCAo model), and immediately followed with treatment by tail-vein injection. After 24 hrs, mouse brains were examined with viability dye TTC (red). 1A shows coronal brain slice from untreated mouse 24 hours with large area of necrotic tissue (green dashed outline). 1B shows brain from treated mouse at 24 hours with a significant reduction in lesion size. 1C shows histogram of average lesion size calculated from 4 mice per group.

Purinergic Treatment at 24 hours Post-Thrombosis Decreases the Size of Cortical Lesions

Mice were imaged at day 0 (pre-thrombosis) and at 24, 48 hours post-thrombosis. At 24 hours, mice were treated via tail vein injection. 2A displays summary of 5 control and 5 treated mice demonstrating significant improvement even at late intervention time points.

Lesion Area Graph

For further detail, see: Zheng, W., Talley Watts, L., Holstein, D.M., Wewer, J., and Lechleiter, J.D. Purinergic receptor stimulation reduces cytotoxic edema and brain infarcts in mouse induced by photothrombosis by energizing glial mitochondria. PLoS One 5, e14401 (2010).

Developing innovative neuroprotection therapies for Traumatic Brain Injuries (TBI), concussions, stroke, and neurodegenerative disorders